Roads were blocked with rocks and burning tyres across Gatueng.
Johannesburg Metro Police said it is related to the motion of no confidence in President Jacob Zuma, set to take place in Parliament on Tuesday morning.
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The blockades were causing widespread traffic disruptions and motorists were advised to take care and use alternate routes.
Bus services in Soweto have been affected.
Roads are also blocked in Turffontein in southern Johannesburg
In Olievenhoutbosch, the R55 is affected, slowing traffic in Midrand.
Protests have also been reported in Tshwane. Affected areas include Mamelodi, Atteridgeville, and Soshanguve.